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KMID : 0613820190290010069
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Journal of Life Science
2019 Volume.29 No. 1 p.69 ~ p.75
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Evaluation of the Biological Activity Affected by Extracting Solvents of Rosemary (Rosmarinus officinalis L.)
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Li Ke
Yang Kyeong-Hee
Guo Lu
Cui Zheng-Wei
Son Beung-Gu
Kang Jum-Soon
Lee Yong-Jae
Park Young-Hoon
Je Beong-Il
Choi Young-Whan
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Abstract
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Rosemary (Rosmarinus officinalis L.) is widely used as a food material. Although various physiological activities of rosemary have been reported, there have been no studies on the physiological activity of solvent extracts with different polarities. Rosemary extracts were obtained by extraction of dried powder using 0%, 25%, 50%, 70%, and 95% ethanol (EtOH) in distilled water, methanol, ethyl acetate, and hexane. As these ratios of EtOH are generally chosen by default and scarcely optimized, we investigated the impact of the composition of EtOH in distilled water on extract-related characteristics, such as DPPH free radical scavenging and α-glucosidase inhibition, on the differentiation of 3T3-L1 adipocytes and inhibition of tyrosinase. Adipogenesis inhibition was highest at 70% EtOH. DPPH scavenging activity and inhibition of tyrosinase activity were reduced with 50% EtOH in water. However, inhibition of α-glucosidase activity was higher in 50% EtOH in water. The best solvents in terms of DPPH scavenging activity, inhibition of tyrosinase and α-glucosidase, and differentiation of adipocytes obtained with different concentrations of EtOH, although a lower similar activities were found with 50% ethanol. Considering the extraction solvents, a ratio of EtOH in water gives different content and constituents of compounds. These differences will give activities inhibition of adipogenesis, tyrosinase, α-glucosidase activity, and DPPH scavenging activity.
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KEYWORD
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Anti-obesity, anti-oxidant, extracts, Rosmarinus officinalis L., solvent selection
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